Melissa Cramer

Dynamic Laboratory Manager with a proven track record at Charles River, excelling in product development and process improvement. Expert in method validation and cross-functional collaboration, driving high-quality outcomes. Recognized for leading successful continuous improvement projects, enhancing operational efficiency, and delivering impactful results in laboratory settings.

• Lossie, AC, Baumgarner KM, Cramer MJ, Garner JP, Justice MJ, 2012, ENU mutagenesis reveals that Notchless homolog 1 (Drosophila) affects Cdkn1a and several members of the Wnt pathway during murine pre-implantation development., BMC Genet, 13, 106
• Lo CL, Shen F, Baumgarner K, Cramer MJ, Lossie AC, 2011, Identification of 129S6/SvEvTac-specific polymorphisms on mouse chromosome 11., DNA Cell Biol, 31, 402-414
• Cramer MJ, Haghshenas N, Bagwell CE, Matsui GY, Lovell CR, 2010, Celerinatantimonas diazitrophica gen. nov., sp. nov., a nitrogen fixing bacterium representing a new family in the Gamma proteobacteria., Int J Syst Evol Microbiol, 61, 1053-1060

Design & Validation of Endotoxin Detection methods and supporting materials: Kinetic Chromogenic Analysis (KCA), Kinetic Turbidimetric Analysis (KTA), Endosafe Cartridge Systems, Gel Clot, Design & Validation of DNA Detection Methods, Genetic Testing for Low Level Presence (LLP)/Adventitious Presence (AP), DNA & RNA Extraction & Quantification, Sterility, bioburden, endotoxin, growth promotion and environmental monitoring testing, TaqMan PCR, Real Time PCR, Reverse Transcription & Sequencing, Nucleic Acid Analysis using Northern & Southern Blotting Techniques, Restriction Enzyme Digestion & Sodium Bisulfite treatment of DNA, Bacterial Characterization: Aseptic Technique, Media Preparation, Anaerobic Chamber Techniques, Transformation, Strain Cultivation, Ligation, Cloning & Denatured Gradient Gel Electrophoresis, Protein Analysis using Western Blotting and ELISA techniques, Experimental Design, Data Collection & Statistical Analyses, Synthesis of Data Records & Technical Reports, Quality Control Testing, Root Cause Analysis, Six Sigma (Certified Green Belt) & process improvement, Writing SOPs, URS & Best Practices, ISO 17025, GMP, GDP & Continual Improvement, JMP, LIMS, bespoke databases, Sequencher, eNotebook, Lab Archives, Vector NTI, Pilgrim, Graph Pad, EndoScan, Minitab, Lightcycler 480, Spectrophotometer, PheraStar, OmegaStar, KingFisher, Endosafe, Lumiscan, Multi-Cartridge System (MCS) Endotoxin Cartridge Reader, Portable Test System (PTS) Endotoxin Cartridge Reader, Bio-Tek Microplate Reader, Epoch 2 Plate Reader, Cellometer, Automation Experience: ViiA7 with Twister Arm, Beckman liquid handlers (MC & NX), Meridian, & MultiFlo liquid dispensers, Hydrocycler, Nexus robotic system

Analytical, highly adaptable Microbiologist/Molecular Biologist with over 15 years of industry and academia experience. Skilled in experimental design, data collection and statistical analyses. Consistent track record of success with quality control and quality assurance testing. Accomplished communicator skilled in building and strengthening relationships across functions to drive cohesive, strategic operations.